End-to-End Genomic Services
Each service is optimized for maximum data quality and minimum turnaround time. Our experienced scientists work closely with you from experimental design through final data delivery.
Sanger DNA Sequencing
Our flagship service provides gold-standard capillary electrophoresis sequencing using Applied Biosystems 3730xl DNA Analyzers. We routinely achieve read lengths exceeding 900 bases with Phred quality scores above Q20 across the entire read. Ideal for plasmid verification, mutation confirmation, and genotyping applications.
- Read lengths up to 1,100 bases per reaction
- Both premixed and plate-based submissions accepted
- Forward and reverse sequencing with consensus assembly
- Free primer stocks for common vectors (pUC, pET, pGEM)
- ABI trace files (.ab1) and FASTA delivered electronically
Real-Time PCR (qPCR)
Quantitative PCR services for gene expression analysis, pathogen detection, copy number variation, and transgene quantification. We support both TaqMan probe-based and SYBR Green intercalating dye chemistries on QuantStudio 6 and 7 Pro platforms. Custom assay design and validation services available.
- TaqMan and SYBR Green chemistries supported
- Multiplex assays up to 6 targets per well
- Absolute and relative quantification (ΔΔCt)
- Standard curve generation and efficiency reporting
- High-throughput 384-well plate format available
Fragment Analysis
High-resolution capillary electrophoresis for precise DNA fragment sizing. Our fragment analysis platform supports microsatellite (SSR) genotyping, AFLP fingerprinting, T-RFLP community profiling, MLPA dosage analysis, and LOH/MSI detection. We use GeneScan 500 LIZ and 1200 LIZ size standards for maximum accuracy.
- Sub-nucleotide sizing precision (±0.3 bp)
- Multi-color multiplexing (FAM, VIC, NED, PET, LIZ)
- Automated allele calling with GeneMapper software
- Size range: 35 bp to 1,200 bp
- Binning sets provided for population studies
Next-Generation Sequencing (NGS)
Illumina-based sequencing solutions spanning the full range of NGS applications. We offer complete workflows from nucleic acid extraction and library preparation through sequencing and bioinformatics analysis. Our team has extensive experience with whole genome sequencing, exome capture, RNA-seq, ChIP-seq, ATAC-seq, and 16S/ITS amplicon metagenomics.
- Illumina NovaSeq 6000 and MiSeq platforms
- Library prep: Nextera, TruSeq, and custom protocols
- Bioinformatics: alignment, variant calling, DE analysis
- Data delivery via secure FTP or cloud storage
- FASTQ, BAM, VCF, and publication-ready figures
SNP Genotyping
Targeted single nucleotide polymorphism detection for pharmacogenomics, ancestry studies, and marker-assisted selection. We offer both TaqMan SNP Genotyping Assays and Sanger-based confirmation services. Custom assay design available for novel variants not covered by existing commercial panels.
- Pre-designed TaqMan assays for 10M+ human SNPs
- Custom assay design and validation in 5-7 days
- High-throughput OpenArray format (up to 3,072 per run)
- Sanger confirmation for critical variants
- Hardy-Weinberg equilibrium testing included
Cell-Based Assays
Comprehensive cell line characterization services including STR profiling for authentication, mycoplasma testing by qPCR, and cell viability/cytotoxicity assays. We follow ATCC, ICLAC, and ANSI/ATCC ASN-0002 standards for all cell line identity testing. Essential for journal submission compliance and grant applications.
- 16-locus STR profiling with amelogenin
- Mycoplasma detection (sensitivity: 1 CFU/mL)
- Comparison against ATCC, DSMZ, and JCRB databases
- Certificate of Authentication for publications
- Species identification by CO1 barcoding